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dvl2 phospho-ser298 rabbit polyclonal antibody  (ProSci Incorporated)


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    ProSci Incorporated dvl2 phospho-ser298 rabbit polyclonal antibody
    A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and <t>DVL2-FLAG</t> were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.
    Dvl2 Phospho Ser298 Rabbit Polyclonal Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dvl2+phospho+ser298/dvl2+phospho+ser298+rabbit+polyclonal+antibody/pmc04094295-188-3-11
    Average 90 stars, based on 1 article reviews
    dvl2 phospho-ser298 rabbit polyclonal antibody - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "RIPK4 phosphorylates Dishevelled proteins to regulate canonical Wnt signaling"

    Article Title: RIPK4 phosphorylates Dishevelled proteins to regulate canonical Wnt signaling

    Journal: Science (New York, N.Y.)

    doi: 10.1126/science.1232253

    A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and DVL2-FLAG were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.
    Figure Legend Snippet: A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and DVL2-FLAG were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.

    Techniques Used: Transfection, Control, In Vitro, Immunoprecipitation, Mutagenesis, Plasmid Preparation, Immunofluorescence, Microscopy

    Related Articles

    Generated:

    Article Title: RIPK4 phosphorylates Dishevelled proteins to regulate canonical Wnt signaling
    Article Snippet: Antibodies recognized RIPK4 (Novus clone 2G3), actin, PKC-δ, DVL2, DVL3, phospho-LRP6-Ser1490, LRP6, β-catenin, GSK3-β, or AXIN1 (Cell Signaling Technology), GSK3 (Millipore), HA or FLAG (Sigma), Myc (US Biologicals), DVL1 or DVL2 (Santa Cruz Biotech), and PKC-β (BD Biosciences). .. DVL2 phospho-Ser298 and DVL2 phospho-Ser480 rabbit polyclonal antibodies were generated by Prosci, and monoclonal antibodies were then derived by Epitomics and Genentech. .. Other reagents were Wnt3a (Genentech and R & D systems), anti-FLAG and anti-HA beads (Sigma), calf intestinal alkaline phosphatase and all restriction enzymes (New England Biolabs).

    Derivative Assay:

    Article Title: RIPK4 phosphorylates Dishevelled proteins to regulate canonical Wnt signaling
    Article Snippet: Antibodies recognized RIPK4 (Novus clone 2G3), actin, PKC-δ, DVL2, DVL3, phospho-LRP6-Ser1490, LRP6, β-catenin, GSK3-β, or AXIN1 (Cell Signaling Technology), GSK3 (Millipore), HA or FLAG (Sigma), Myc (US Biologicals), DVL1 or DVL2 (Santa Cruz Biotech), and PKC-β (BD Biosciences). .. DVL2 phospho-Ser298 and DVL2 phospho-Ser480 rabbit polyclonal antibodies were generated by Prosci, and monoclonal antibodies were then derived by Epitomics and Genentech. .. Other reagents were Wnt3a (Genentech and R & D systems), anti-FLAG and anti-HA beads (Sigma), calf intestinal alkaline phosphatase and all restriction enzymes (New England Biolabs).



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    dvl2 phospho-ser298 rabbit polyclonal antibody  (ProSci Incorporated)
    90
    ProSci Incorporated dvl2 phospho-ser298 rabbit polyclonal antibody
    A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and <t>DVL2-FLAG</t> were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.
    Dvl2 Phospho Ser298 Rabbit Polyclonal Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dvl2+phospho+ser298/dvl2+phospho+ser298+rabbit+polyclonal+antibody/pmc04094295-188-3-11
    Average 90 stars, based on 1 article reviews
    dvl2 phospho-ser298 rabbit polyclonal antibody - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    dvl2 phospho ser298  (ProSci Incorporated)
    90
    ProSci Incorporated dvl2 phospho ser298
    A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and <t>DVL2-FLAG</t> were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.
    Dvl2 Phospho Ser298, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dvl2+phospho+ser298/DVL2+Antibody/pmc04094295-94-0-11
    Average 90 stars, based on 1 article reviews
    dvl2 phospho ser298 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and DVL2-FLAG were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.

    Journal: Science (New York, N.Y.)

    Article Title: RIPK4 phosphorylates Dishevelled proteins to regulate canonical Wnt signaling

    doi: 10.1126/science.1232253

    Figure Lengend Snippet: A. HEK293T cells were transfected with control (Ctrl) or RIPK4 siRNAs for 72 h and then given vehicle or 200 ng/mL Wnt3a for 30 min. B. RIPK4-Myc and DVL2-FLAG were expressed with rabbit reticulocyte and wheat germ in vitro translation systems, respectively. The proteins were mixed and immunoprecipitated with anti-FLAG beads. C. HEK293T cells treated with 200 ng/mL Wnt3a. D. In vitro kinase assays using the RIPK4 kinase domain (wild-type or K51R mutant) and FLAG-tagged DEP or PDZ domains of DVL2. E. HEK293T cells transfected with control or RIPK4 siRNA for 72 h, and then given vehicle or 200 ng/mL Wnt3a for 10 min. Non-specific bands (*). F. Dvl-null MEFs reconstituted with empty vector or DVL2 (wild-type or mutant S298A S480A) and treated with Wnt3a. G. Immunofluorescence microscopy of HeLa cells transfected with RIPK4-GFP and DVL2-FLAG for 36 h. Cells containing DVL2 puncta (signalosome) were enumerated by counting 250 cells per condition. Scale bars: 10 μm.

    Article Snippet: DVL2 phospho-Ser298 and DVL2 phospho-Ser480 rabbit polyclonal antibodies were generated by Prosci, and monoclonal antibodies were then derived by Epitomics and Genentech.

    Techniques: Transfection, Control, In Vitro, Immunoprecipitation, Mutagenesis, Plasmid Preparation, Immunofluorescence, Microscopy